Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice

Emmanuel Asuquo Etim; Yusuf Abdulhakeem Adebayo; Obeagu Emmanuel Ifeanyi

doi:doi:10.11648/j.wjph.20190404.14

| Peer-Reviewed

Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice

Emmanuel Asuquo Etim, Yusuf Abdulhakeem Adebayo, Obeagu Emmanuel Ifeanyi

Published in World Journal of Public Health (Volume 4, Issue 4)

Received: 9 May 2018 Accepted: 24 May 2018 Published: 10 December 2019

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Background/Objective: Achyranthes aspera is an erect flowering medicinal herb use in folkloric medicine. This study aims to examine the effect of methanolic leaf extract of Achyranthes aspera on hematological parameters of Swiss albino mice. Materials and Methods: 70 adult mice of both sexes with mean weight of 22 ± 5g were used for this study. Achyranthes aspera leaves was pulverized to coarse powder from where methanolic extract was obtained. 0.2ml of 50mg/kg, 100mg/kg, and 200mg/kg respectively of leaf extract was administered to mice for four days. After administration, 1ml of blood was collected from mice through cardiac puncture into EDTA bottle for full blood count estimation using Abacus-80 hematological analyzer. Results: The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract resulted in increased lymphocyte count from 29.4±7.35% to 70.4±0.25%, 70.5±0.219% and 81.3±0.282% respectively at P>0.05 while neutrophil value was reduced from 55.2±11.05 x 10⁹/l to 14.0±0.216x 10⁹/l, 11.2±0.109x10⁹/l and 12.3±0.148 x10⁹/l. The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract increases platelet count from 99.0±0.52x10³/ul to 529±0.01x 10³/ul, 332±0.523x10³/ul and 135±0.543x10³/ul respectively. Conclusion: Ingestion of methanolic leaf extract of Achyranthes aspera resulted in a dose dependent changes in hematological parameter of albino mice including significant increase in lymphocyte and platelet counts. This plant’s leaf extract may therefore have the potentials of being effective in the treatment of diseases cause by thrombocytopenia and lymphocytopenia in mammals. It is believed that information provided in this study can enhance appropriate usage of Achyranthes aspera in folkloric medicine in Nigeria.

Published in	World Journal of Public Health (Volume 4, Issue 4)
DOI	10.11648/j.wjph.20190404.14
Page(s)	96-101
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Achyranthes Aspera, Hematological Parameter, Albino Mice

References

[1]	Geetha K. Antidiabetic activity of Achyranthes aspera L. with alloxanised mice for the estimation of level of glucose and cholesterol. Asian J. Plant Sci. Res. 2016; 6 (2): 18-23.
[2]	Aziz A, Rahman M, Mondal AK. 3-Acetoxy-6-benzoyloxyapagamide from Achyranthes aspera, Pharmaceutical J. 2005; 4: 1816-1820.
[3]	S. Edwin, E. Jarald, D. L. Edwin, A. Jain, H. Kinger, K. R. Dutt, A. A. Raj. Wound healing and antioxidant activity of Achyranthes aspera. Pharmaceutical Biol. 2008; 46 (12), 824-828.
[4]	N. G. Sutar, U. N. Sutar, Y. P. Sharma, I. K. Shaikh, S. S. Kshirsagar. Phytochemical investigation and pharmacological screening of Achyranthes aspera Lnn as analgesic and antipyretic. Biosci Biotech Res Asia. 2008; 5 (2): 841-844.
[5]	B. R. Goyal, S. G. Mahajan, R. G. Mali, R. K. Goyal, A. A. Mehta. Hypoglycemic Effect of Achyranthes aspera L. Methanol Extract and Fractions on Streptozotocin-induced Diabetic Rats. Global J Pharmacolol. 2007; 1 (1): 6-12.
[6]	C. C. Barua, A. Talukdar, S. A. Begum, B. Buragohain, J. D. Roy, R. S. Borah, M. Lahkar. Antidepresant-like effect of the methanolic extract of Achyranthes aspera Lnn in animal’s models of depression. Pharmacologyonline, 2009; 2: 587-594.
[7]	Saurabh Srivastav, Pradeep Singh, Garima Mishra, K. K. Jha, R. L. Khosa. Achyranthes aspera-An important medicinal plant: A review. J. Nat. Prod. Plant Resour. 2011; 1 (1): 1-14.
[8]	Chakraborty Asima, Adelheid Brantner, Teruo Mukainaka, Yoshitoshi Nobukuni, Masahi Kuchide, Takao Konoshima, Harukuni Tokuda, Hoyoku Nishino. Cancer chemopreventive activity of Achyranthes aspera leaves on Esptein-Barr virus activation and two-stage mouse skin carcinogenesis. Cancer Letters. 2002; 177: 1-5.
[9]	R. Chakrabarti, R. Y. Vasudeva. Achyranthes aspera stimulates the immunity and enhances the antigen clearance in catla catla. Intl Immunopharmacol. 2006; 6 (5): 782-790.
[10]	A. Bagavan, A. A. Rahuman, C. Kamaraj, K. Geetha. Larvicidal efficacy of five cucurbitaceous plant leaf extract against mosquito species. Parasitol res. 2008; 103 (1): 223-229.
[11]	Victoria O. A, C. A. Elusiyan, F. O. Olorunmola, F. B. Adewoyin, N. O. Omisore A. O. Adepiti J. M. Agbedahunsi and C. O. Adewunmi. Evaluation of antitrypanosomal and anti-inflammatory activities of selected Nigerian medicinal plant in mice. Afr J Tradit Complement Altern Med. 2013; 10 (6): 469-476.
[12]	Ratra PS, Misra KC. Seasonal variation in chemical composition of Achyranthes aspera Linn. and A. bidentata Bl. Indian Forester. 1970; 96 (5): 372-375.
[13]	National Institute of Science Communication and Information Resources. The wealth of India, raw materials: a ready reckoner on biodiversity and bioresources of India. New Delhi: National Institute; 1985.
[14]	A. A. Zahir, A. A. Rahuman, C. Kamaraj, A. Bagavan, G. Elango, A. Sangaran, B. S. Kumar. Parasitic effect of Achyranthes aspera. Parasitol Res. 2009; 105 (2): 453-461.
[15]	A. R. Bafna, S. H. Mishra. Effect of methanol extract of achyranthes aspera linn. on rifampicin-induced hepatotoxicity in rats. Ars Pharmaceutica, 2004; 45 (4): 343-351.
[16]	Belayhun M, Seid T, Yinebeb T, Argaw A. Molluscicidal effect of Achyranthes aspera L. (Amaranthaceae) aqueous extract on adult snails of Biomphalaria pfeifferi and Lymnaea natalensis. Infectious Dis of Poverty. 2017; 6: 133.
[17]	Thilagavathi G, Kannaian T. Application of Pricklychaff (Achyranthes aspera Linn.) leaves as herbal antimicrobial finish for cotton fabric used in healthcare textiles. Natural Pro Rad. 2008; 7: 330-334.
[18]	Vetrichelvan T, Jegadeesan M. Effect of alcohol extract of Achyranthes aspera Linn. on acute and sub-acute inflammation. Phytother Res. 2003; 17: 77-79.
[19]	D. S. Gayathri, A. Archanah, P. Abiramasundari, V. Priya, K. Uma, T. Abirami. Antioxidant activity of commonly used vegetables. Indian J Nutr Diet. 2009; 46 (12), 485-490.
[20]	C. C. Barua, S. A. Begum, A. Talukdar, D. C. Pathak, A. G. Barua, P. Borah, M. Lahkar. effect of achyranthes aspera linn on modified forced swimming in rats. Pharmacologyonline. 2010; 1: 183-191.
[21]	S. B. Datir, A. B. Ganjare, S. A. Nirmal, S. B. Bhawar, D. K. Bharati, M. J. Patil. Pharmacologyonline. 2009; 921-925.
[22]	Vasudeva N, Sharma SK. Post-coital antifertility activity of Achyranthes aspera Linn root. J Ethnopharmacol. 2006; 107: 179-181.
[23]	Shibeshi W, Makonnen E, Debella A, Zerihun L. Phytochemical, contraceptive efficacy and safety Evaluations of the MeOH leaves extract of Achyranthes aspera L. in rats. Pharmacologyonline. 2006; 3: 217-224.
[24]	Shibeshi W, Makonnen E, Zerihun L, Debella A. Effect of Achyranthes aspera L. on fetal abortion, uterine and pituitary weights, serum lipids and hormones. Afr Health Sc. 2006; 6 (2): 108-112.
[25]	Bassey EO, Essien EE, Ching FP, Mbagwu HO. An Evaluation of the Sub Acute Toxicity and Haemostatic Effects of Leaves Extract of Achyranthes aspera in Mice and Albino Rats. EJMP.2015; 7 (1): 16-25.
[26]	S. K. Sharma, N. Vasudeva, M. Ali. Indian Journal of Chemistry - Section B Organic and Medicinal Chemistry. 2009; 48 (8), 1164-1169.
[27]	Ram P. Rastogi, B. N. Mehrotra. Compendium of Indian Medicinal plants. Central Drug Research Institute, Lucknow and National institute of science communication and information resources, New Delhi. 2004; 5: 7-8, 11.
[28]	Kamble A, Reddy C, Patil S. Testicular Activity of Mice Treated with MeOH Extract of Achyranthes aspera Leaves. J Adv Med Sc and Applied Technol. 2017; 3 (2); 93-100.
[29]	Y. Tijani, M. O. Uguru, O. A. Salawu. Anti-Pyretic, Anti-Inflammatory and Anti-Diarrhoel Properties of Faidherbia Albida in Rats. Afr J Biotechnol, 2008, 7 (6), 696-700.
[30]	Trease, GC and Evans, WC. (1989) textbook of pharmacology 13th ed. ELBS, Bailliere Tindall, London, pp. 683-684.
[31]	Harbone J. B, (1983) phytochemical methods. A guide to modern technique of plant analysis. Chapman and Hall, New York. Pp. 49-188.
[32]	Dawit, D, Eyassu, M, Asfwa, D, Dawi, A, Kelbessa, U, Walleligen, M. In vivo anti-malaria activity of hydroalcoholic extract from Asparagus africanus Lam in mice infected with Plasmodium berghei. Ethiop J Health Dev. 2006; 20 (2): 112-118.
[33]	Victoria, CU, Ebele, AE, Obina, IE, Theophine, CO, Godwin, CA, Chukwuemeka, M. U. Antimalaria activity of aqueous exract and fraction of leaves of Agerantum conyzoides in mice infected with P. bergei. Int. J Pharmaceutical Sc. 2010; 2 (1): 33-38.
[34]	Chung KT, Wong TY, Wei CI, Huang YW, Lin Y. Tannins and human health: a review. Crit Rev Food Sci Nutr. 1998; 38 (6): 421-464.
[35]	Aniagu SO, Nwinyi FC, Akumka DD, Agbani EO, Dzama S, Ajoku GA, Izebe KS, Agala P, Adelusola KA, Ibe J, Uford I, Gamaniel KS Short-term toxicity studies of Ficus thonningii leaf extract in rats. Int. J. Food Technol. 2008; 43 (3): 456-463.
[36]	Lang E, Qadri SM, Lang F. Killing me softly: suicidal eryth-rocyte death. Int J Biochem Cell Biol. 2012; 44: 1236–1243.
[37]	Etim EA, Emokpae MA, Medugu JT, Medugu FI. Correlation of lymphocyte count with serum calcium level and neutrophil-to-lymphocyte ratio in end stage renal disease patients undergoing hemodialysis in Adamawa State, Nigeria. Int. J. Med. Med. Sci. 2017; 9 (5): 47-50.
[38]	R. S. Bachhav, R. Sambathkumar. Evaluation of Immunomodulatory Activity of the Alkaloid Fraction of Trichopus zeylanicus Gaertn on Experimental Animals. Indian J Pharm Sci. 2016; 78 (1): 161–166.

Cite This Article

Plain Text BibTeX RIS

APA Style

Emmanuel Asuquo Etim, Yusuf Abdulhakeem Adebayo, Obeagu Emmanuel Ifeanyi. (2019). Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice. World Journal of Public Health, 4(4), 96-101. https://doi.org/10.11648/j.wjph.20190404.14

Copy | Download

ACS Style

Emmanuel Asuquo Etim; Yusuf Abdulhakeem Adebayo; Obeagu Emmanuel Ifeanyi. Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice. World J. Public Health 2019, 4(4), 96-101. doi: 10.11648/j.wjph.20190404.14

Copy | Download

AMA Style

Emmanuel Asuquo Etim, Yusuf Abdulhakeem Adebayo, Obeagu Emmanuel Ifeanyi. Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice. World J Public Health. 2019;4(4):96-101. doi: 10.11648/j.wjph.20190404.14

Copy | Download

@article{10.11648/j.wjph.20190404.14,
  author = {Emmanuel Asuquo Etim and Yusuf Abdulhakeem Adebayo and Obeagu Emmanuel Ifeanyi},
  title = {Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice},
  journal = {World Journal of Public Health},
  volume = {4},
  number = {4},
  pages = {96-101},
  doi = {10.11648/j.wjph.20190404.14},
  url = {https://doi.org/10.11648/j.wjph.20190404.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjph.20190404.14},
  abstract = {Background/Objective: Achyranthes aspera is an erect flowering medicinal herb use in folkloric medicine. This study aims to examine the effect of methanolic leaf extract of Achyranthes aspera on hematological parameters of Swiss albino mice. Materials and Methods: 70 adult mice of both sexes with mean weight of 22 ± 5g were used for this study. Achyranthes aspera leaves was pulverized to coarse powder from where methanolic extract was obtained. 0.2ml of 50mg/kg, 100mg/kg, and 200mg/kg respectively of leaf extract was administered to mice for four days. After administration, 1ml of blood was collected from mice through cardiac puncture into EDTA bottle for full blood count estimation using Abacus-80 hematological analyzer. Results: The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract resulted in increased lymphocyte count from 29.4±7.35% to 70.4±0.25%, 70.5±0.219% and 81.3±0.282% respectively at P>0.05 while neutrophil value was reduced from 55.2±11.05 x 109/l to 14.0±0.216x 109/l, 11.2±0.109x109/l and 12.3±0.148 x109/l. The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract increases platelet count from 99.0±0.52x103/ul to 529±0.01x 103/ul, 332±0.523x103/ul and 135±0.543x103/ul respectively. Conclusion: Ingestion of methanolic leaf extract of Achyranthes aspera resulted in a dose dependent changes in hematological parameter of albino mice including significant increase in lymphocyte and platelet counts. This plant’s leaf extract may therefore have the potentials of being effective in the treatment of diseases cause by thrombocytopenia and lymphocytopenia in mammals. It is believed that information provided in this study can enhance appropriate usage of Achyranthes aspera in folkloric medicine in Nigeria.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice
AU  - Emmanuel Asuquo Etim
AU  - Yusuf Abdulhakeem Adebayo
AU  - Obeagu Emmanuel Ifeanyi
Y1  - 2019/12/10
PY  - 2019
N1  - https://doi.org/10.11648/j.wjph.20190404.14
DO  - 10.11648/j.wjph.20190404.14
T2  - World Journal of Public Health
JF  - World Journal of Public Health
JO  - World Journal of Public Health
SP  - 96
EP  - 101
PB  - Science Publishing Group
SN  - 2637-6059
UR  - https://doi.org/10.11648/j.wjph.20190404.14
AB  - Background/Objective: Achyranthes aspera is an erect flowering medicinal herb use in folkloric medicine. This study aims to examine the effect of methanolic leaf extract of Achyranthes aspera on hematological parameters of Swiss albino mice. Materials and Methods: 70 adult mice of both sexes with mean weight of 22 ± 5g were used for this study. Achyranthes aspera leaves was pulverized to coarse powder from where methanolic extract was obtained. 0.2ml of 50mg/kg, 100mg/kg, and 200mg/kg respectively of leaf extract was administered to mice for four days. After administration, 1ml of blood was collected from mice through cardiac puncture into EDTA bottle for full blood count estimation using Abacus-80 hematological analyzer. Results: The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract resulted in increased lymphocyte count from 29.4±7.35% to 70.4±0.25%, 70.5±0.219% and 81.3±0.282% respectively at P>0.05 while neutrophil value was reduced from 55.2±11.05 x 109/l to 14.0±0.216x 109/l, 11.2±0.109x109/l and 12.3±0.148 x109/l. The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract increases platelet count from 99.0±0.52x103/ul to 529±0.01x 103/ul, 332±0.523x103/ul and 135±0.543x103/ul respectively. Conclusion: Ingestion of methanolic leaf extract of Achyranthes aspera resulted in a dose dependent changes in hematological parameter of albino mice including significant increase in lymphocyte and platelet counts. This plant’s leaf extract may therefore have the potentials of being effective in the treatment of diseases cause by thrombocytopenia and lymphocytopenia in mammals. It is believed that information provided in this study can enhance appropriate usage of Achyranthes aspera in folkloric medicine in Nigeria.
VL  - 4
IS  - 4
ER  -

Copy | Download

Author Information

Emmanuel Asuquo Etim

Department of Laboratory Services, Federal Medical Centre, Yola, Adamawa State, Nigeria
Yusuf Abdulhakeem Adebayo

Department of Laboratory Services, Federal Medical Centre, Yola, Adamawa State, Nigeria
Obeagu Emmanuel Ifeanyi

Department of University Health Services, Micheal Okpara University, Abia State, Nigeria

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Emmanuel Asuquo Etim, Yusuf Abdulhakeem Adebayo, Obeagu Emmanuel Ifeanyi. (2019). Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice. World Journal of Public Health, 4(4), 96-101. https://doi.org/10.11648/j.wjph.20190404.14

Copy | Download

ACS Style

Emmanuel Asuquo Etim; Yusuf Abdulhakeem Adebayo; Obeagu Emmanuel Ifeanyi. Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice. World J. Public Health 2019, 4(4), 96-101. doi: 10.11648/j.wjph.20190404.14

Copy | Download

AMA Style

Emmanuel Asuquo Etim, Yusuf Abdulhakeem Adebayo, Obeagu Emmanuel Ifeanyi. Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice. World J Public Health. 2019;4(4):96-101. doi: 10.11648/j.wjph.20190404.14

Copy | Download

@article{10.11648/j.wjph.20190404.14,
  author = {Emmanuel Asuquo Etim and Yusuf Abdulhakeem Adebayo and Obeagu Emmanuel Ifeanyi},
  title = {Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice},
  journal = {World Journal of Public Health},
  volume = {4},
  number = {4},
  pages = {96-101},
  doi = {10.11648/j.wjph.20190404.14},
  url = {https://doi.org/10.11648/j.wjph.20190404.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjph.20190404.14},
  abstract = {Background/Objective: Achyranthes aspera is an erect flowering medicinal herb use in folkloric medicine. This study aims to examine the effect of methanolic leaf extract of Achyranthes aspera on hematological parameters of Swiss albino mice. Materials and Methods: 70 adult mice of both sexes with mean weight of 22 ± 5g were used for this study. Achyranthes aspera leaves was pulverized to coarse powder from where methanolic extract was obtained. 0.2ml of 50mg/kg, 100mg/kg, and 200mg/kg respectively of leaf extract was administered to mice for four days. After administration, 1ml of blood was collected from mice through cardiac puncture into EDTA bottle for full blood count estimation using Abacus-80 hematological analyzer. Results: The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract resulted in increased lymphocyte count from 29.4±7.35% to 70.4±0.25%, 70.5±0.219% and 81.3±0.282% respectively at P>0.05 while neutrophil value was reduced from 55.2±11.05 x 109/l to 14.0±0.216x 109/l, 11.2±0.109x109/l and 12.3±0.148 x109/l. The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract increases platelet count from 99.0±0.52x103/ul to 529±0.01x 103/ul, 332±0.523x103/ul and 135±0.543x103/ul respectively. Conclusion: Ingestion of methanolic leaf extract of Achyranthes aspera resulted in a dose dependent changes in hematological parameter of albino mice including significant increase in lymphocyte and platelet counts. This plant’s leaf extract may therefore have the potentials of being effective in the treatment of diseases cause by thrombocytopenia and lymphocytopenia in mammals. It is believed that information provided in this study can enhance appropriate usage of Achyranthes aspera in folkloric medicine in Nigeria.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice
AU  - Emmanuel Asuquo Etim
AU  - Yusuf Abdulhakeem Adebayo
AU  - Obeagu Emmanuel Ifeanyi
Y1  - 2019/12/10
PY  - 2019
N1  - https://doi.org/10.11648/j.wjph.20190404.14
DO  - 10.11648/j.wjph.20190404.14
T2  - World Journal of Public Health
JF  - World Journal of Public Health
JO  - World Journal of Public Health
SP  - 96
EP  - 101
PB  - Science Publishing Group
SN  - 2637-6059
UR  - https://doi.org/10.11648/j.wjph.20190404.14
AB  - Background/Objective: Achyranthes aspera is an erect flowering medicinal herb use in folkloric medicine. This study aims to examine the effect of methanolic leaf extract of Achyranthes aspera on hematological parameters of Swiss albino mice. Materials and Methods: 70 adult mice of both sexes with mean weight of 22 ± 5g were used for this study. Achyranthes aspera leaves was pulverized to coarse powder from where methanolic extract was obtained. 0.2ml of 50mg/kg, 100mg/kg, and 200mg/kg respectively of leaf extract was administered to mice for four days. After administration, 1ml of blood was collected from mice through cardiac puncture into EDTA bottle for full blood count estimation using Abacus-80 hematological analyzer. Results: The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract resulted in increased lymphocyte count from 29.4±7.35% to 70.4±0.25%, 70.5±0.219% and 81.3±0.282% respectively at P>0.05 while neutrophil value was reduced from 55.2±11.05 x 109/l to 14.0±0.216x 109/l, 11.2±0.109x109/l and 12.3±0.148 x109/l. The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract increases platelet count from 99.0±0.52x103/ul to 529±0.01x 103/ul, 332±0.523x103/ul and 135±0.543x103/ul respectively. Conclusion: Ingestion of methanolic leaf extract of Achyranthes aspera resulted in a dose dependent changes in hematological parameter of albino mice including significant increase in lymphocyte and platelet counts. This plant’s leaf extract may therefore have the potentials of being effective in the treatment of diseases cause by thrombocytopenia and lymphocytopenia in mammals. It is believed that information provided in this study can enhance appropriate usage of Achyranthes aspera in folkloric medicine in Nigeria.
VL  - 4
IS  - 4
ER  -

Copy | Download