Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis

Wei Song; Wei Chen; Ya’nan Yang; Caiyan Li; Guoying Qian

doi:doi:10.11648/j.ijnfs.20140304.16

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Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis

Wei Song, Wei Chen, Ya’nan Yang, Caiyan Li, Guoying Qian

Published in International Journal of Nutrition and Food Sciences (Volume 3, Issue 4)

Received: 5 June 2014 Accepted: 20 June 2014 Published: 30 June 2014

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Abstract

The soft-shelled turtle (Pelodiscus sinensis) is a commercially important aquatic species with abundant collagen content and precious nutritious high quality goods. The present study was to optimize the collagen extraction from lung of Pelodiscus sinensis. Single-factor test was employed to investigate the effects of different extraction methods and the major factors that influence the collagen production in enzymatic extraction method on the collagen yield of lung. Optimization of the papain enzymatic extraction parameters was then examined using an orthogonal test design L9 (34). The optimum extraction conditions were obtained when the extraction temperature, papain enzyme dose, the ratio of solid to solution, and extraction time were 32°C, 4.0mg/ml, 1:35, and 12h, respectively. Under the optimized conditions, the collagen yield was up to 79.29%. The properties of turtle lung collagen were characterized by SDS-PAGE, UV scanning, and amino acid analysis. The results showed that the extracted lung collagen had high imino acid content at 21.8% and contained two α chains, β, and γ-components, belonging to the typical type I collagen. The amphibious aquatic turtle collagen is thought to be a viable novel source for replacement of terrestrial mammals and could provide scientific reference for the development of collagen polypeptide for terrapin animals.

Published in	International Journal of Nutrition and Food Sciences (Volume 3, Issue 4)
DOI	10.11648/j.ijnfs.20140304.16
Page(s)	270-278
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Soft-shelled turtle Pelodiscus sinensis, Collagen extraction, Optimization, Characterization

References

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APA Style

Wei Song, Wei Chen, Ya’nan Yang, Caiyan Li, Guoying Qian. (2014). Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis. International Journal of Nutrition and Food Sciences, 3(4), 270-278. https://doi.org/10.11648/j.ijnfs.20140304.16

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ACS Style

Wei Song; Wei Chen; Ya’nan Yang; Caiyan Li; Guoying Qian. Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis. Int. J. Nutr. Food Sci. 2014, 3(4), 270-278. doi: 10.11648/j.ijnfs.20140304.16

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AMA Style

Wei Song, Wei Chen, Ya’nan Yang, Caiyan Li, Guoying Qian. Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis. Int J Nutr Food Sci. 2014;3(4):270-278. doi: 10.11648/j.ijnfs.20140304.16

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@article{10.11648/j.ijnfs.20140304.16,
  author = {Wei Song and Wei Chen and Ya’nan Yang and Caiyan Li and Guoying Qian},
  title = {Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {3},
  number = {4},
  pages = {270-278},
  doi = {10.11648/j.ijnfs.20140304.16},
  url = {https://doi.org/10.11648/j.ijnfs.20140304.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20140304.16},
  abstract = {The soft-shelled turtle (Pelodiscus sinensis) is a commercially important aquatic species with abundant collagen content and precious nutritious high quality goods. The present study was to optimize the collagen extraction from lung of Pelodiscus sinensis. Single-factor test was employed to investigate the effects of different extraction methods and the major factors that influence the collagen production in enzymatic extraction method on the collagen yield of lung. Optimization of the papain enzymatic extraction parameters was then examined using an orthogonal test design L9 (34). The optimum extraction conditions were obtained when the extraction temperature, papain enzyme dose, the ratio of solid to solution, and extraction time were 32°C, 4.0mg/ml, 1:35, and 12h, respectively. Under the optimized conditions, the collagen yield was up to 79.29%. The properties of turtle lung collagen were characterized by SDS-PAGE, UV scanning, and amino acid analysis. The results showed that the extracted lung collagen had high imino acid content at 21.8% and contained two α chains, β, and γ-components, belonging to the typical type I collagen. The amphibious aquatic turtle collagen is thought to be a viable novel source for replacement of terrestrial mammals and could provide scientific reference for the development of collagen polypeptide for terrapin animals.},
 year = {2014}
}

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TY  - JOUR
T1  - Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis
AU  - Wei Song
AU  - Wei Chen
AU  - Ya’nan Yang
AU  - Caiyan Li
AU  - Guoying Qian
Y1  - 2014/06/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ijnfs.20140304.16
DO  - 10.11648/j.ijnfs.20140304.16
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 270
EP  - 278
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20140304.16
AB  - The soft-shelled turtle (Pelodiscus sinensis) is a commercially important aquatic species with abundant collagen content and precious nutritious high quality goods. The present study was to optimize the collagen extraction from lung of Pelodiscus sinensis. Single-factor test was employed to investigate the effects of different extraction methods and the major factors that influence the collagen production in enzymatic extraction method on the collagen yield of lung. Optimization of the papain enzymatic extraction parameters was then examined using an orthogonal test design L9 (34). The optimum extraction conditions were obtained when the extraction temperature, papain enzyme dose, the ratio of solid to solution, and extraction time were 32°C, 4.0mg/ml, 1:35, and 12h, respectively. Under the optimized conditions, the collagen yield was up to 79.29%. The properties of turtle lung collagen were characterized by SDS-PAGE, UV scanning, and amino acid analysis. The results showed that the extracted lung collagen had high imino acid content at 21.8% and contained two α chains, β, and γ-components, belonging to the typical type I collagen. The amphibious aquatic turtle collagen is thought to be a viable novel source for replacement of terrestrial mammals and could provide scientific reference for the development of collagen polypeptide for terrapin animals.
VL  - 3
IS  - 4
ER  -

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Author Information

Wei Song

College of Biological and Environmental Sciences, Zhejiang Wanli University, South Qianhu Road 8, Ningbo 315100, China
Wei Chen

College of Biological and Environmental Sciences, Zhejiang Wanli University, South Qianhu Road 8, Ningbo 315100, China
Ya’nan Yang

College of Biological and Environmental Sciences, Zhejiang Wanli University, South Qianhu Road 8, Ningbo 315100, China
Caiyan Li

College of Biological and Environmental Sciences, Zhejiang Wanli University, South Qianhu Road 8, Ningbo 315100, China
Guoying Qian

College of Biological and Environmental Sciences, Zhejiang Wanli University, South Qianhu Road 8, Ningbo 315100, China

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APA Style

Wei Song, Wei Chen, Ya’nan Yang, Caiyan Li, Guoying Qian. (2014). Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis. International Journal of Nutrition and Food Sciences, 3(4), 270-278. https://doi.org/10.11648/j.ijnfs.20140304.16

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ACS Style

Wei Song; Wei Chen; Ya’nan Yang; Caiyan Li; Guoying Qian. Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis. Int. J. Nutr. Food Sci. 2014, 3(4), 270-278. doi: 10.11648/j.ijnfs.20140304.16

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AMA Style

Wei Song, Wei Chen, Ya’nan Yang, Caiyan Li, Guoying Qian. Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis. Int J Nutr Food Sci. 2014;3(4):270-278. doi: 10.11648/j.ijnfs.20140304.16

Copy | Download

@article{10.11648/j.ijnfs.20140304.16,
  author = {Wei Song and Wei Chen and Ya’nan Yang and Caiyan Li and Guoying Qian},
  title = {Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {3},
  number = {4},
  pages = {270-278},
  doi = {10.11648/j.ijnfs.20140304.16},
  url = {https://doi.org/10.11648/j.ijnfs.20140304.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20140304.16},
  abstract = {The soft-shelled turtle (Pelodiscus sinensis) is a commercially important aquatic species with abundant collagen content and precious nutritious high quality goods. The present study was to optimize the collagen extraction from lung of Pelodiscus sinensis. Single-factor test was employed to investigate the effects of different extraction methods and the major factors that influence the collagen production in enzymatic extraction method on the collagen yield of lung. Optimization of the papain enzymatic extraction parameters was then examined using an orthogonal test design L9 (34). The optimum extraction conditions were obtained when the extraction temperature, papain enzyme dose, the ratio of solid to solution, and extraction time were 32°C, 4.0mg/ml, 1:35, and 12h, respectively. Under the optimized conditions, the collagen yield was up to 79.29%. The properties of turtle lung collagen were characterized by SDS-PAGE, UV scanning, and amino acid analysis. The results showed that the extracted lung collagen had high imino acid content at 21.8% and contained two α chains, β, and γ-components, belonging to the typical type I collagen. The amphibious aquatic turtle collagen is thought to be a viable novel source for replacement of terrestrial mammals and could provide scientific reference for the development of collagen polypeptide for terrapin animals.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis
AU  - Wei Song
AU  - Wei Chen
AU  - Ya’nan Yang
AU  - Caiyan Li
AU  - Guoying Qian
Y1  - 2014/06/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ijnfs.20140304.16
DO  - 10.11648/j.ijnfs.20140304.16
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 270
EP  - 278
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20140304.16
AB  - The soft-shelled turtle (Pelodiscus sinensis) is a commercially important aquatic species with abundant collagen content and precious nutritious high quality goods. The present study was to optimize the collagen extraction from lung of Pelodiscus sinensis. Single-factor test was employed to investigate the effects of different extraction methods and the major factors that influence the collagen production in enzymatic extraction method on the collagen yield of lung. Optimization of the papain enzymatic extraction parameters was then examined using an orthogonal test design L9 (34). The optimum extraction conditions were obtained when the extraction temperature, papain enzyme dose, the ratio of solid to solution, and extraction time were 32°C, 4.0mg/ml, 1:35, and 12h, respectively. Under the optimized conditions, the collagen yield was up to 79.29%. The properties of turtle lung collagen were characterized by SDS-PAGE, UV scanning, and amino acid analysis. The results showed that the extracted lung collagen had high imino acid content at 21.8% and contained two α chains, β, and γ-components, belonging to the typical type I collagen. The amphibious aquatic turtle collagen is thought to be a viable novel source for replacement of terrestrial mammals and could provide scientific reference for the development of collagen polypeptide for terrapin animals.
VL  - 3
IS  - 4
ER  -

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