Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E

Alceu Afonso Jordao; Thais Helena Monteiro; Raquel Alves dos Santos; Guilherme Vannucchi Portari

doi:doi:10.11648/j.ajbls.20170505.11

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Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E

Alceu Afonso Jordao, Thais Helena Monteiro, Raquel Alves dos Santos, Guilherme Vannucchi Portari

Published in American Journal of Biomedical and Life Sciences (Volume 5, Issue 5)

Received: 11 April 2017 Accepted: 23 May 2017 Published: 18 October 2017

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Abstract

The metabolism of ethanol is directly related to oxidative stress, and its ingestion leads to the formation of reactive oxygen species (ROS) such as hydroxyl radicals, superoxide and hydrogen peroxide. Vitamin E has been widely used as an antioxidant; when administered in large doses it is deposited in the liver and then excreted in the bile, urine and feces. The objective of the present study was to evaluate the rate of excretion of fecal vitamin E in relation to its concentrations in serum and liver, and its role as a protective antioxidant against DNA damage induced by acute ethanol consumption. Wistar rats were divided into four groups receiving food and water ad libitum for 4 days plus the following treatments: Control (CG, n = 10 ) no treatment; Ethanol (ET, n = 10 ), receiving an acute ethanol dose intraperitoneally in the amount of 5 g/kg; vitamin E (VE, n = 10) receiving a high oral dose of vitamin E within the first three days in the amount of 100 tocopherol mg/kg body weight; ethanol plus vitamin E (VE + ET, n = 10 ) receiving both the ethanol and vitamin E doses. Higher concentrations of vitamin E were observed in the blood and liver of the animals in the groups that received vitamin E supplementation, independent of the presence or absence of ethanol. Concomitantly, these groups were also those with the highest concentration of the vitamin in the stool. The rate of DNA damage was higher in the groups that received ethanol with or without supplemental vitamin E. However, the rate of damage was lower in the group that received vitamin E supplementation than in the group that did not. The present results show that vitamin E has a protective effect against DNA damage induced by ethanol by reducing the extent of DNA damage.

Published in	American Journal of Biomedical and Life Sciences (Volume 5, Issue 5)
DOI	10.11648/j.ajbls.20170505.11
Page(s)	88-91
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Vitamin E, Ethanol, Antioxidants, DNA Damage, Supplementation

References

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[19]	Tavares DC, Cecchi AO, Jordão Jr AA, Vannucchi H, Takahashi CS. Cytogenetic study of chronic ethanol consumption in rats. Teratogenesis, Carcinogenesis, and Mutagenesis 2001; 21: 361-8.
[20]	Lee SJ, Kim SY, Min H. Effects of vitamin C and E supplementation on oxidative stress and liver toxicity in rats fed a low-fat ethanol diet. Nutr Res Pract. 2013; 7: 109-114.
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Alceu Afonso Jordao, Thais Helena Monteiro, Raquel Alves dos Santos, Guilherme Vannucchi Portari. (2017). Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E. American Journal of Biomedical and Life Sciences, 5(5), 88-91. https://doi.org/10.11648/j.ajbls.20170505.11

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ACS Style

Alceu Afonso Jordao; Thais Helena Monteiro; Raquel Alves dos Santos; Guilherme Vannucchi Portari. Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E. Am. J. Biomed. Life Sci. 2017, 5(5), 88-91. doi: 10.11648/j.ajbls.20170505.11

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AMA Style

Alceu Afonso Jordao, Thais Helena Monteiro, Raquel Alves dos Santos, Guilherme Vannucchi Portari. Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E. Am J Biomed Life Sci. 2017;5(5):88-91. doi: 10.11648/j.ajbls.20170505.11

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@article{10.11648/j.ajbls.20170505.11,
  author = {Alceu Afonso Jordao and Thais Helena Monteiro and Raquel Alves dos Santos and Guilherme Vannucchi Portari},
  title = {Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {5},
  number = {5},
  pages = {88-91},
  doi = {10.11648/j.ajbls.20170505.11},
  url = {https://doi.org/10.11648/j.ajbls.20170505.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20170505.11},
  abstract = {The metabolism of ethanol is directly related to oxidative stress, and its ingestion leads to the formation of reactive oxygen species (ROS) such as hydroxyl radicals, superoxide and hydrogen peroxide. Vitamin E has been widely used as an antioxidant; when administered in large doses it is deposited in the liver and then excreted in the bile, urine and feces. The objective of the present study was to evaluate the rate of excretion of fecal vitamin E in relation to its concentrations in serum and liver, and its role as a protective antioxidant against DNA damage induced by acute ethanol consumption. Wistar rats were divided into four groups receiving food and water ad libitum for 4 days plus the following treatments: Control (CG, n = 10 ) no treatment; Ethanol (ET, n = 10 ), receiving an acute ethanol dose intraperitoneally in the amount of 5 g/kg; vitamin E (VE, n = 10) receiving a high oral dose of vitamin E within the first three days in the amount of 100 tocopherol mg/kg body weight; ethanol plus vitamin E (VE + ET, n = 10 ) receiving both the ethanol and vitamin E doses. Higher concentrations of vitamin E were observed in the blood and liver of the animals in the groups that received vitamin E supplementation, independent of the presence or absence of ethanol. Concomitantly, these groups were also those with the highest concentration of the vitamin in the stool. The rate of DNA damage was higher in the groups that received ethanol with or without supplemental vitamin E. However, the rate of damage was lower in the group that received vitamin E supplementation than in the group that did not. The present results show that vitamin E has a protective effect against DNA damage induced by ethanol by reducing the extent of DNA damage.},
 year = {2017}
}

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TY  - JOUR
T1  - Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E
AU  - Alceu Afonso Jordao
AU  - Thais Helena Monteiro
AU  - Raquel Alves dos Santos
AU  - Guilherme Vannucchi Portari
Y1  - 2017/10/18
PY  - 2017
N1  - https://doi.org/10.11648/j.ajbls.20170505.11
DO  - 10.11648/j.ajbls.20170505.11
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 88
EP  - 91
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20170505.11
AB  - The metabolism of ethanol is directly related to oxidative stress, and its ingestion leads to the formation of reactive oxygen species (ROS) such as hydroxyl radicals, superoxide and hydrogen peroxide. Vitamin E has been widely used as an antioxidant; when administered in large doses it is deposited in the liver and then excreted in the bile, urine and feces. The objective of the present study was to evaluate the rate of excretion of fecal vitamin E in relation to its concentrations in serum and liver, and its role as a protective antioxidant against DNA damage induced by acute ethanol consumption. Wistar rats were divided into four groups receiving food and water ad libitum for 4 days plus the following treatments: Control (CG, n = 10 ) no treatment; Ethanol (ET, n = 10 ), receiving an acute ethanol dose intraperitoneally in the amount of 5 g/kg; vitamin E (VE, n = 10) receiving a high oral dose of vitamin E within the first three days in the amount of 100 tocopherol mg/kg body weight; ethanol plus vitamin E (VE + ET, n = 10 ) receiving both the ethanol and vitamin E doses. Higher concentrations of vitamin E were observed in the blood and liver of the animals in the groups that received vitamin E supplementation, independent of the presence or absence of ethanol. Concomitantly, these groups were also those with the highest concentration of the vitamin in the stool. The rate of DNA damage was higher in the groups that received ethanol with or without supplemental vitamin E. However, the rate of damage was lower in the group that received vitamin E supplementation than in the group that did not. The present results show that vitamin E has a protective effect against DNA damage induced by ethanol by reducing the extent of DNA damage.
VL  - 5
IS  - 5
ER  -

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Author Information

Alceu Afonso Jordao

Division of Nutrition, Department of Internal Medicine, Medical School of Ribeir?o Preto, University of S?o Paulo, Ribeir?o Preto, Brazil
Thais Helena Monteiro

Division of Nutrition, Department of Internal Medicine, Medical School of Ribeir?o Preto, University of S?o Paulo, Ribeir?o Preto, Brazil
Raquel Alves dos Santos

Nucleus of Research in Sciences and Technology, University of Franca, Franca, Brazil
Guilherme Vannucchi Portari

Department of Nutrition, Federal University of Triangulo Mineiro, Uberaba, Brazil

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APA Style

Alceu Afonso Jordao, Thais Helena Monteiro, Raquel Alves dos Santos, Guilherme Vannucchi Portari. (2017). Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E. American Journal of Biomedical and Life Sciences, 5(5), 88-91. https://doi.org/10.11648/j.ajbls.20170505.11

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ACS Style

Alceu Afonso Jordao; Thais Helena Monteiro; Raquel Alves dos Santos; Guilherme Vannucchi Portari. Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E. Am. J. Biomed. Life Sci. 2017, 5(5), 88-91. doi: 10.11648/j.ajbls.20170505.11

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AMA Style

Alceu Afonso Jordao, Thais Helena Monteiro, Raquel Alves dos Santos, Guilherme Vannucchi Portari. Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E. Am J Biomed Life Sci. 2017;5(5):88-91. doi: 10.11648/j.ajbls.20170505.11

Copy | Download

@article{10.11648/j.ajbls.20170505.11,
  author = {Alceu Afonso Jordao and Thais Helena Monteiro and Raquel Alves dos Santos and Guilherme Vannucchi Portari},
  title = {Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {5},
  number = {5},
  pages = {88-91},
  doi = {10.11648/j.ajbls.20170505.11},
  url = {https://doi.org/10.11648/j.ajbls.20170505.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20170505.11},
  abstract = {The metabolism of ethanol is directly related to oxidative stress, and its ingestion leads to the formation of reactive oxygen species (ROS) such as hydroxyl radicals, superoxide and hydrogen peroxide. Vitamin E has been widely used as an antioxidant; when administered in large doses it is deposited in the liver and then excreted in the bile, urine and feces. The objective of the present study was to evaluate the rate of excretion of fecal vitamin E in relation to its concentrations in serum and liver, and its role as a protective antioxidant against DNA damage induced by acute ethanol consumption. Wistar rats were divided into four groups receiving food and water ad libitum for 4 days plus the following treatments: Control (CG, n = 10 ) no treatment; Ethanol (ET, n = 10 ), receiving an acute ethanol dose intraperitoneally in the amount of 5 g/kg; vitamin E (VE, n = 10) receiving a high oral dose of vitamin E within the first three days in the amount of 100 tocopherol mg/kg body weight; ethanol plus vitamin E (VE + ET, n = 10 ) receiving both the ethanol and vitamin E doses. Higher concentrations of vitamin E were observed in the blood and liver of the animals in the groups that received vitamin E supplementation, independent of the presence or absence of ethanol. Concomitantly, these groups were also those with the highest concentration of the vitamin in the stool. The rate of DNA damage was higher in the groups that received ethanol with or without supplemental vitamin E. However, the rate of damage was lower in the group that received vitamin E supplementation than in the group that did not. The present results show that vitamin E has a protective effect against DNA damage induced by ethanol by reducing the extent of DNA damage.},
 year = {2017}
}

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TY  - JOUR
T1  - Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E
AU  - Alceu Afonso Jordao
AU  - Thais Helena Monteiro
AU  - Raquel Alves dos Santos
AU  - Guilherme Vannucchi Portari
Y1  - 2017/10/18
PY  - 2017
N1  - https://doi.org/10.11648/j.ajbls.20170505.11
DO  - 10.11648/j.ajbls.20170505.11
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 88
EP  - 91
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20170505.11
AB  - The metabolism of ethanol is directly related to oxidative stress, and its ingestion leads to the formation of reactive oxygen species (ROS) such as hydroxyl radicals, superoxide and hydrogen peroxide. Vitamin E has been widely used as an antioxidant; when administered in large doses it is deposited in the liver and then excreted in the bile, urine and feces. The objective of the present study was to evaluate the rate of excretion of fecal vitamin E in relation to its concentrations in serum and liver, and its role as a protective antioxidant against DNA damage induced by acute ethanol consumption. Wistar rats were divided into four groups receiving food and water ad libitum for 4 days plus the following treatments: Control (CG, n = 10 ) no treatment; Ethanol (ET, n = 10 ), receiving an acute ethanol dose intraperitoneally in the amount of 5 g/kg; vitamin E (VE, n = 10) receiving a high oral dose of vitamin E within the first three days in the amount of 100 tocopherol mg/kg body weight; ethanol plus vitamin E (VE + ET, n = 10 ) receiving both the ethanol and vitamin E doses. Higher concentrations of vitamin E were observed in the blood and liver of the animals in the groups that received vitamin E supplementation, independent of the presence or absence of ethanol. Concomitantly, these groups were also those with the highest concentration of the vitamin in the stool. The rate of DNA damage was higher in the groups that received ethanol with or without supplemental vitamin E. However, the rate of damage was lower in the group that received vitamin E supplementation than in the group that did not. The present results show that vitamin E has a protective effect against DNA damage induced by ethanol by reducing the extent of DNA damage.
VL  - 5
IS  - 5
ER  -

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