Synergistic Evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica with Antibiotics Against Environmental MRSA Isolates: An In-vitro Study

Akinrotoye Kehinde Peter; Akinduti Paul; Lanlokun Olabisi; Adetogun Clement

doi:doi:10.11648/j.ajbio.20200804.11

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Synergistic Evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica with Antibiotics Against Environmental MRSA Isolates: An In-vitro Study

Akinrotoye Kehinde Peter, Akinduti Paul, Lanlokun Olabisi, Adetogun Clement

Published in American Journal of BioScience (Volume 8, Issue 4)

Received: 25 May 2020 Accepted: 8 June 2020 Published: 6 July 2020

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Abstract

Methicillin–Resistant Staphylococcus aureus (MRSA) is known to show resistance to beta-lactam class of antibiotics. MRSA is among the highest superbugs posing dangerous threats to humans. This study aimed at determining the in-vitro synergistic evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica extracts with existing antibiotics (Azithromycin, Clindamycin and Vancomycin) on isolated MRSA from fomites. MRSA was isolated using the BBL™ Oxacillin agar screen test (Müller Hinton Agar with 6 μg/mL Oxacillin and 4% NaCl). The Minimum Bactericidal concentration (MBC) of the MRSA were determined by Agar well diffusion using antibiotics in solitary, plant extracts in solitary, combination of these antibiotics with plant extracts at different concentrations. The agar diffusion assay showed that H. umbellate extract-Azithromycin combination had the least zones of inhibition ≥21.00±1.92 mm in 75% of all isolates testedwhile M. oleifera extract-Azithromycin combination had the highest zones of inhibition ≥22.20 ±2.27 mm. Comparison of bactericidal activities of all plant extracts and antibiotics synergy shows Azithromycin to have a significant value of P>0.05. The agar well diffusion method showed synergistic effects between combination of antibiotics and all extracts with significant increase in the zones of inhibition of the test antibiotics against environmental strains of MRSA. The synergistic interactions indicated that the inhibitory potentials of the plant extracts increased hence, combining natural products derived from phytochemicals and antibiotics could be another way to mitigate and fight against resistant infectious bacteria.

Published in	American Journal of BioScience (Volume 8, Issue 4)
DOI	10.11648/j.ajbio.20200804.11
Page(s)	91-98
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Antimicrobial Resistance, MRSA, Antibiotics, Plant Extracts, Synergism

References

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Akinrotoye Kehinde Peter, Akinduti Paul, Lanlokun Olabisi, Adetogun Clement. (2020). Synergistic Evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica with Antibiotics Against Environmental MRSA Isolates: An In-vitro Study. American Journal of BioScience, 8(4), 91-98. https://doi.org/10.11648/j.ajbio.20200804.11

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ACS Style

Akinrotoye Kehinde Peter; Akinduti Paul; Lanlokun Olabisi; Adetogun Clement. Synergistic Evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica with Antibiotics Against Environmental MRSA Isolates: An In-vitro Study. Am. J. BioScience 2020, 8(4), 91-98. doi: 10.11648/j.ajbio.20200804.11

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AMA Style

Akinrotoye Kehinde Peter, Akinduti Paul, Lanlokun Olabisi, Adetogun Clement. Synergistic Evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica with Antibiotics Against Environmental MRSA Isolates: An In-vitro Study. Am J BioScience. 2020;8(4):91-98. doi: 10.11648/j.ajbio.20200804.11

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@article{10.11648/j.ajbio.20200804.11,
  author = {Akinrotoye Kehinde Peter and Akinduti Paul and Lanlokun Olabisi and Adetogun Clement},
  title = {Synergistic Evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica with Antibiotics Against Environmental MRSA Isolates: An In-vitro Study},
  journal = {American Journal of BioScience},
  volume = {8},
  number = {4},
  pages = {91-98},
  doi = {10.11648/j.ajbio.20200804.11},
  url = {https://doi.org/10.11648/j.ajbio.20200804.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20200804.11},
  abstract = {Methicillin–Resistant Staphylococcus aureus (MRSA) is known to show resistance to beta-lactam class of antibiotics. MRSA is among the highest superbugs posing dangerous threats to humans. This study aimed at determining the in-vitro synergistic evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica extracts with existing antibiotics (Azithromycin, Clindamycin and Vancomycin) on isolated MRSA from fomites. MRSA was isolated using the BBL™ Oxacillin agar screen test (Müller Hinton Agar with 6 μg/mL Oxacillin and 4% NaCl). The Minimum Bactericidal concentration (MBC) of the MRSA were determined by Agar well diffusion using antibiotics in solitary, plant extracts in solitary, combination of these antibiotics with plant extracts at different concentrations. The agar diffusion assay showed that H. umbellate extract-Azithromycin combination had the least zones of inhibition ≥21.00±1.92 mm in 75% of all isolates testedwhile M. oleifera extract-Azithromycin combination had the highest zones of inhibition ≥22.20 ±2.27 mm. Comparison of bactericidal activities of all plant extracts and antibiotics synergy shows Azithromycin to have a significant value of P>0.05. The agar well diffusion method showed synergistic effects between combination of antibiotics and all extracts with significant increase in the zones of inhibition of the test antibiotics against environmental strains of MRSA. The synergistic interactions indicated that the inhibitory potentials of the plant extracts increased hence, combining natural products derived from phytochemicals and antibiotics could be another way to mitigate and fight against resistant infectious bacteria.},
 year = {2020}
}

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TY  - JOUR
T1  - Synergistic Evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica with Antibiotics Against Environmental MRSA Isolates: An In-vitro Study
AU  - Akinrotoye Kehinde Peter
AU  - Akinduti Paul
AU  - Lanlokun Olabisi
AU  - Adetogun Clement
Y1  - 2020/07/06
PY  - 2020
N1  - https://doi.org/10.11648/j.ajbio.20200804.11
DO  - 10.11648/j.ajbio.20200804.11
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 91
EP  - 98
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20200804.11
AB  - Methicillin–Resistant Staphylococcus aureus (MRSA) is known to show resistance to beta-lactam class of antibiotics. MRSA is among the highest superbugs posing dangerous threats to humans. This study aimed at determining the in-vitro synergistic evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica extracts with existing antibiotics (Azithromycin, Clindamycin and Vancomycin) on isolated MRSA from fomites. MRSA was isolated using the BBL™ Oxacillin agar screen test (Müller Hinton Agar with 6 μg/mL Oxacillin and 4% NaCl). The Minimum Bactericidal concentration (MBC) of the MRSA were determined by Agar well diffusion using antibiotics in solitary, plant extracts in solitary, combination of these antibiotics with plant extracts at different concentrations. The agar diffusion assay showed that H. umbellate extract-Azithromycin combination had the least zones of inhibition ≥21.00±1.92 mm in 75% of all isolates testedwhile M. oleifera extract-Azithromycin combination had the highest zones of inhibition ≥22.20 ±2.27 mm. Comparison of bactericidal activities of all plant extracts and antibiotics synergy shows Azithromycin to have a significant value of P>0.05. The agar well diffusion method showed synergistic effects between combination of antibiotics and all extracts with significant increase in the zones of inhibition of the test antibiotics against environmental strains of MRSA. The synergistic interactions indicated that the inhibitory potentials of the plant extracts increased hence, combining natural products derived from phytochemicals and antibiotics could be another way to mitigate and fight against resistant infectious bacteria.
VL  - 8
IS  - 4
ER  -

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Author Information

Akinrotoye Kehinde Peter

Department of Microbiology, College of Biosciences, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
Akinduti Paul

Department of Biological Sciences, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria
Lanlokun Olabisi

Department of Microbiology, College of Biosciences, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
Adetogun Clement

Department of Forestry and Wildlife Management, College of Environmental Resources and Management, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria

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Akinrotoye Kehinde Peter, Akinduti Paul, Lanlokun Olabisi, Adetogun Clement. (2020). Synergistic Evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica with Antibiotics Against Environmental MRSA Isolates: An In-vitro Study. American Journal of BioScience, 8(4), 91-98. https://doi.org/10.11648/j.ajbio.20200804.11

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Akinrotoye Kehinde Peter; Akinduti Paul; Lanlokun Olabisi; Adetogun Clement. Synergistic Evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica with Antibiotics Against Environmental MRSA Isolates: An In-vitro Study. Am. J. BioScience 2020, 8(4), 91-98. doi: 10.11648/j.ajbio.20200804.11

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AMA Style

Akinrotoye Kehinde Peter, Akinduti Paul, Lanlokun Olabisi, Adetogun Clement. Synergistic Evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica with Antibiotics Against Environmental MRSA Isolates: An In-vitro Study. Am J BioScience. 2020;8(4):91-98. doi: 10.11648/j.ajbio.20200804.11

Copy | Download

@article{10.11648/j.ajbio.20200804.11,
  author = {Akinrotoye Kehinde Peter and Akinduti Paul and Lanlokun Olabisi and Adetogun Clement},
  title = {Synergistic Evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica with Antibiotics Against Environmental MRSA Isolates: An In-vitro Study},
  journal = {American Journal of BioScience},
  volume = {8},
  number = {4},
  pages = {91-98},
  doi = {10.11648/j.ajbio.20200804.11},
  url = {https://doi.org/10.11648/j.ajbio.20200804.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20200804.11},
  abstract = {Methicillin–Resistant Staphylococcus aureus (MRSA) is known to show resistance to beta-lactam class of antibiotics. MRSA is among the highest superbugs posing dangerous threats to humans. This study aimed at determining the in-vitro synergistic evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica extracts with existing antibiotics (Azithromycin, Clindamycin and Vancomycin) on isolated MRSA from fomites. MRSA was isolated using the BBL™ Oxacillin agar screen test (Müller Hinton Agar with 6 μg/mL Oxacillin and 4% NaCl). The Minimum Bactericidal concentration (MBC) of the MRSA were determined by Agar well diffusion using antibiotics in solitary, plant extracts in solitary, combination of these antibiotics with plant extracts at different concentrations. The agar diffusion assay showed that H. umbellate extract-Azithromycin combination had the least zones of inhibition ≥21.00±1.92 mm in 75% of all isolates testedwhile M. oleifera extract-Azithromycin combination had the highest zones of inhibition ≥22.20 ±2.27 mm. Comparison of bactericidal activities of all plant extracts and antibiotics synergy shows Azithromycin to have a significant value of P>0.05. The agar well diffusion method showed synergistic effects between combination of antibiotics and all extracts with significant increase in the zones of inhibition of the test antibiotics against environmental strains of MRSA. The synergistic interactions indicated that the inhibitory potentials of the plant extracts increased hence, combining natural products derived from phytochemicals and antibiotics could be another way to mitigate and fight against resistant infectious bacteria.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - Synergistic Evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica with Antibiotics Against Environmental MRSA Isolates: An In-vitro Study
AU  - Akinrotoye Kehinde Peter
AU  - Akinduti Paul
AU  - Lanlokun Olabisi
AU  - Adetogun Clement
Y1  - 2020/07/06
PY  - 2020
N1  - https://doi.org/10.11648/j.ajbio.20200804.11
DO  - 10.11648/j.ajbio.20200804.11
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 91
EP  - 98
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20200804.11
AB  - Methicillin–Resistant Staphylococcus aureus (MRSA) is known to show resistance to beta-lactam class of antibiotics. MRSA is among the highest superbugs posing dangerous threats to humans. This study aimed at determining the in-vitro synergistic evaluation of Moringa oleifera, Hunteria umbellate and Azadirachta indica extracts with existing antibiotics (Azithromycin, Clindamycin and Vancomycin) on isolated MRSA from fomites. MRSA was isolated using the BBL™ Oxacillin agar screen test (Müller Hinton Agar with 6 μg/mL Oxacillin and 4% NaCl). The Minimum Bactericidal concentration (MBC) of the MRSA were determined by Agar well diffusion using antibiotics in solitary, plant extracts in solitary, combination of these antibiotics with plant extracts at different concentrations. The agar diffusion assay showed that H. umbellate extract-Azithromycin combination had the least zones of inhibition ≥21.00±1.92 mm in 75% of all isolates testedwhile M. oleifera extract-Azithromycin combination had the highest zones of inhibition ≥22.20 ±2.27 mm. Comparison of bactericidal activities of all plant extracts and antibiotics synergy shows Azithromycin to have a significant value of P>0.05. The agar well diffusion method showed synergistic effects between combination of antibiotics and all extracts with significant increase in the zones of inhibition of the test antibiotics against environmental strains of MRSA. The synergistic interactions indicated that the inhibitory potentials of the plant extracts increased hence, combining natural products derived from phytochemicals and antibiotics could be another way to mitigate and fight against resistant infectious bacteria.
VL  - 8
IS  - 4
ER  -

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