A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis

Hrachya Garegin Hovhannisyan; Gohar Gagik Grigoryan

doi:doi:10.11648/j.ajbio.20140203.11

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A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis

Hrachya Garegin Hovhannisyan, Gohar Gagik Grigoryan

Published in American Journal of BioScience (Volume 2, Issue 3)

Received: 7 April 2014 Accepted: 15 April 2014 Published: 30 April 2014

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Abstract

Various factors; nutrition, hygiene, stress, infections, low immunity, menses, frequent sexual intercourses, pregnancy etc., destroyed vaginal microbial balance, which reflected in the number of lactobacilli decrease and increase the number of pathogenic microorganisms, as well as the pH-value. All this in turn causes vaginosis. The aim of this study is creation of symbiotic association of probiotic bacteria (SAPB) with high colonization/recolonization properties for rapid response to vaginal acute and chronic challenges. It is expected that LABs symbiotic association will be more beneficial for vaginal colonization/recoclonization, because the lactococci are growth well at elevate pH 9.0-5.0 and by reducing of vaginal pH promote the growth of lactobacilli, adapted to low pH 6.5-3.5. In this study used lactobacilli biocompatible with cocci possessing high antimicrobial activity from laboratory LABs collection, isolated earlier from 20-45 years old healthy Ar-menian woman volunteers (n=40). And it is the first time that lactococci were used as probiotic in vaginal coloniza-tion/recolonization practice. The symbiotic association of Lactobacillus plantarum (L. plantarum) GH 202 and Lactococcus lactis (L. lactis) GH 204 strains shows higher inhibitory activities against bacterial pathogens and Candida albicans (C. albicans). The SAPB growth rate is more intensive and biomass accumulation is higher, than the mono cultures. It is sustainable at different pH conditions of growth and during multiple subculturing imitating up and down changes of vaginal pH. The SAPB strains have high hydrophobicity evaluated by MATS test and high coaggregation properties essential for increasing their colonization potential, when they used in mixed culture.

Published in	American Journal of BioScience (Volume 2, Issue 3)
DOI	10.11648/j.ajbio.20140203.11
Page(s)	84-88
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

LABs Association, Antimicrobial Activity, Adhesion, Coaggregation

References

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Hrachya Garegin Hovhannisyan, Gohar Gagik Grigoryan. (2014). A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis. American Journal of BioScience, 2(3), 84-88. https://doi.org/10.11648/j.ajbio.20140203.11

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ACS Style

Hrachya Garegin Hovhannisyan; Gohar Gagik Grigoryan. A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis. Am. J. BioScience 2014, 2(3), 84-88. doi: 10.11648/j.ajbio.20140203.11

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AMA Style

Hrachya Garegin Hovhannisyan, Gohar Gagik Grigoryan. A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis. Am J BioScience. 2014;2(3):84-88. doi: 10.11648/j.ajbio.20140203.11

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@article{10.11648/j.ajbio.20140203.11,
  author = {Hrachya Garegin Hovhannisyan and Gohar Gagik Grigoryan},
  title = {A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis},
  journal = {American Journal of BioScience},
  volume = {2},
  number = {3},
  pages = {84-88},
  doi = {10.11648/j.ajbio.20140203.11},
  url = {https://doi.org/10.11648/j.ajbio.20140203.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140203.11},
  abstract = {Various factors; nutrition, hygiene, stress, infections, low immunity, menses, frequent sexual intercourses, pregnancy etc., destroyed vaginal microbial balance, which reflected in the number of lactobacilli decrease and increase the number of pathogenic microorganisms, as well as the pH-value. All this in turn causes vaginosis. The aim of this study is creation of symbiotic association of probiotic bacteria (SAPB) with high colonization/recolonization properties for rapid response to vaginal acute and chronic challenges. It is expected that LABs symbiotic association will be more beneficial for vaginal colonization/recoclonization, because the lactococci are growth well at elevate pH 9.0-5.0 and by reducing of vaginal pH promote the growth of lactobacilli, adapted to low pH 6.5-3.5. In this study used lactobacilli biocompatible with cocci possessing high antimicrobial activity from laboratory LABs collection, isolated earlier from 20-45 years old healthy Ar-menian woman volunteers (n=40). And it is the first time that lactococci were used as probiotic in vaginal coloniza-tion/recolonization practice. The symbiotic association of Lactobacillus plantarum (L. plantarum) GH 202 and Lactococcus lactis (L. lactis) GH 204 strains shows higher inhibitory activities against bacterial pathogens and Candida albicans (C. albicans). The SAPB growth rate is more intensive and biomass accumulation is higher, than the mono cultures. It is sustainable at different pH conditions of growth and during multiple subculturing imitating up and down changes of vaginal pH. The SAPB strains have high hydrophobicity evaluated by MATS test and high coaggregation properties essential for increasing their colonization potential, when they used in mixed culture.},
 year = {2014}
}

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TY - JOUR
T1 - A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis
AU - Hrachya Garegin Hovhannisyan
AU - Gohar Gagik Grigoryan
Y1 - 2014/04/30
PY - 2014
N1 - https://doi.org/10.11648/j.ajbio.20140203.11
DO - 10.11648/j.ajbio.20140203.11
T2 - American Journal of BioScience
JF - American Journal of BioScience
JO - American Journal of BioScience
SP - 84
EP - 88
PB - Science Publishing Group
SN - 2330-0167
UR - https://doi.org/10.11648/j.ajbio.20140203.11
AB - Various factors; nutrition, hygiene, stress, infections, low immunity, menses, frequent sexual intercourses, pregnancy etc., destroyed vaginal microbial balance, which reflected in the number of lactobacilli decrease and increase the number of pathogenic microorganisms, as well as the pH-value. All this in turn causes vaginosis. The aim of this study is creation of symbiotic association of probiotic bacteria (SAPB) with high colonization/recolonization properties for rapid response to vaginal acute and chronic challenges. It is expected that LABs symbiotic association will be more beneficial for vaginal colonization/recoclonization, because the lactococci are growth well at elevate pH 9.0-5.0 and by reducing of vaginal pH promote the growth of lactobacilli, adapted to low pH 6.5-3.5. In this study used lactobacilli biocompatible with cocci possessing high antimicrobial activity from laboratory LABs collection, isolated earlier from 20-45 years old healthy Ar-menian woman volunteers (n=40). And it is the first time that lactococci were used as probiotic in vaginal coloniza-tion/recolonization practice. The symbiotic association of Lactobacillus plantarum (L. plantarum) GH 202 and Lactococcus lactis (L. lactis) GH 204 strains shows higher inhibitory activities against bacterial pathogens and Candida albicans (C. albicans). The SAPB growth rate is more intensive and biomass accumulation is higher, than the mono cultures. It is sustainable at different pH conditions of growth and during multiple subculturing imitating up and down changes of vaginal pH. The SAPB strains have high hydrophobicity evaluated by MATS test and high coaggregation properties essential for increasing their colonization potential, when they used in mixed culture.
VL - 2
IS - 3
ER -

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Author Information

Hrachya Garegin Hovhannisyan

“Armbiotechnology” S&P Center of National Academy of Sciences of Armenia, Yerevan, Armenia
Gohar Gagik Grigoryan

“Armbiotechnology” S&P Center of National Academy of Sciences of Armenia, Yerevan, Armenia

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Hrachya Garegin Hovhannisyan, Gohar Gagik Grigoryan. (2014). A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis. American Journal of BioScience, 2(3), 84-88. https://doi.org/10.11648/j.ajbio.20140203.11

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ACS Style

Hrachya Garegin Hovhannisyan; Gohar Gagik Grigoryan. A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis. Am. J. BioScience 2014, 2(3), 84-88. doi: 10.11648/j.ajbio.20140203.11

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AMA Style

Hrachya Garegin Hovhannisyan, Gohar Gagik Grigoryan. A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis. Am J BioScience. 2014;2(3):84-88. doi: 10.11648/j.ajbio.20140203.11

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@article{10.11648/j.ajbio.20140203.11,
  author = {Hrachya Garegin Hovhannisyan and Gohar Gagik Grigoryan},
  title = {A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis},
  journal = {American Journal of BioScience},
  volume = {2},
  number = {3},
  pages = {84-88},
  doi = {10.11648/j.ajbio.20140203.11},
  url = {https://doi.org/10.11648/j.ajbio.20140203.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140203.11},
  abstract = {Various factors; nutrition, hygiene, stress, infections, low immunity, menses, frequent sexual intercourses, pregnancy etc., destroyed vaginal microbial balance, which reflected in the number of lactobacilli decrease and increase the number of pathogenic microorganisms, as well as the pH-value. All this in turn causes vaginosis. The aim of this study is creation of symbiotic association of probiotic bacteria (SAPB) with high colonization/recolonization properties for rapid response to vaginal acute and chronic challenges. It is expected that LABs symbiotic association will be more beneficial for vaginal colonization/recoclonization, because the lactococci are growth well at elevate pH 9.0-5.0 and by reducing of vaginal pH promote the growth of lactobacilli, adapted to low pH 6.5-3.5. In this study used lactobacilli biocompatible with cocci possessing high antimicrobial activity from laboratory LABs collection, isolated earlier from 20-45 years old healthy Ar-menian woman volunteers (n=40). And it is the first time that lactococci were used as probiotic in vaginal coloniza-tion/recolonization practice. The symbiotic association of Lactobacillus plantarum (L. plantarum) GH 202 and Lactococcus lactis (L. lactis) GH 204 strains shows higher inhibitory activities against bacterial pathogens and Candida albicans (C. albicans). The SAPB growth rate is more intensive and biomass accumulation is higher, than the mono cultures. It is sustainable at different pH conditions of growth and during multiple subculturing imitating up and down changes of vaginal pH. The SAPB strains have high hydrophobicity evaluated by MATS test and high coaggregation properties essential for increasing their colonization potential, when they used in mixed culture.},
 year = {2014}
}

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